She went 0 for 2 on Sunday’s card at Woodbine in Toronto, finishing seventh aboard Crushin Hard in the eighth race, the last ride of her 13-year career.
Sutherland announced her retirement on her website, saying she wants to focus on her new husband and slow down and enjoy her life.
She won 931 races from 7,350 career mounts and had purse earnings of more than $47 million, according to Equibase.
Sutherland became the first female rider to win the Santa Anita Handicap, guiding Game On Dude to a nose victory last year. But she lost the mount on Game On Dude after they were beaten in the Pacific Classic in August at Del Mar and her business had slowed recently.